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Hello all! Stayed at the Excalibur Resort and Casino in Vegas and was pleasantly taken back in time recently. The price for the resort and casino was relatively budget friendly. Enjoy!

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I spent this morning doing slot-blot filter binding assays, which are an experiment in which you measure protein-nucleic acid (DNA or RNA) interactions using a double membrane sandwich with a nitrocellulose membrane on top that traps proteins (and bound nucleic acids) and a nylon membrane below that binds any free nucleic acids. So I planned to make a video showing you how I set it up and run it - and I did - but I also found I had some stuff to say about experimenting in general so I put that stuff first and then the behind-the-scenes look at the blot-a-lot-a-lotting If you want way more details…. much more about slot blotting: http://bit.ly/bindingaffinity more about Kd: http://bit.ly/bindingaffinity more about phosphor storage screens: http://bit.ly/phosphorimaging blog form: https://bit.ly/slotblotalot

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